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This intermediate, termed the Schiff base, can be reduced in vitro by sodium borohydride (NaBH 4 ) treatment, forming an irreversible covalent complex between the enzyme and DNA ( 3 , 13 , 14 ). Apurinic/apyrimidinic (AP) site recognition by the 5'-dRP/AP lyase in poly(ADP-ribose) polymerase-1 (PARP-1) The capacity of human poly(ADP-ribose) polymerase-1 (PARP-1) to interact with intact apurinic/apyrimidinic (AP) sites in DNA has been demonstrated. In cell extracts, sodium borohydride reduction of the PARP-1/AP site DNA complex resulted in (2008) AP lyase. In: Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6754-9_998.RIS DNA-(Apurinic or Apyrimidinic Site) Lyase DNA-Formamidopyrimidine Glycosylase Deoxyguanosine / analogs & derivatives* Catalyzes the excision of an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine = 8-oxoG) from DNA (PubMed:10521423, PubMed:19446526). Also cleaves the DNA backbone at apurinic/apyrimidinic sites (AP sites) (PubMed:10521423, PubMed:19446526).
Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases also possess AP lyase activity that permits them to cut the phosphodiester bond of DNA, creating a single-strand break without the need for an AP endonuclease. Lyaser är en enzymklass vars uppgift är att katalysera spjälkningsreaktioner. Ett lyas bryter en kovalent bindning under bildande av en dubbelbindning; detta till skillnad från ett hydrolas som bryter en kovalent bindning under konsumtion av en vattenmolekyl. (redirected from AP lyase) APEX1 A gene on chromosome 14q11.2 that encodes a major apurinic/apyrimidinic (AP) endoDNAase, which is involved in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents, and in the redox regulation of transcriptional factors. We previously demonstrated mammalian Ku has intrinsic 5' deoxyribosephosphate (5'dRP) and apurinic/apyrimidinic (AP) lyase activity, and showed this activity is important for excising abasic site damage from ends.
Reaction catalysed AP lyase activity of cell extracts with or without Ku. a, b, c, 1 nM AP-DSB at 37°C was incubated with 1 g Mock depleted (Mock), Ku depleted ( -Ku), or Ku depleted + 4 ng recombinant Ku ( -Ku+rKu) human (HeLa) cell extracts, or 10 g parental (K1), Ku deficient (xrs6), or complemented (xrs6+Ku80) rodent (CHO) cell extracts.
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AP synonyms, AP pronunciation, AP translation, English dictionary definition of AP. abbr.
AP lyase activity also was seen in Alkbh1 of Saccharomyces pombe . Schizosaccharomyces pombe AlkB homolog Abh1 exhibits AP lyase activity but no demethylase activity Hanne Korvald a ,b, Pål Ø. Falnesc, Jon K. Laerdahla ,b d, Magnar Bjørås e, Ingrun Alsetha ,b ∗
Proteintech Anti-ATP citrate lyase Polyclonal, Catalog # 15421-1-AP. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 150 µL purified antibody (0.17 mg/mL).
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AP lyase activity of cell extracts with or without Ku. a, b, c, 1 nM AP-DSB at 37°C was incubated with 1 g Mock depleted (Mock), Ku depleted ( -Ku), or Ku depleted + 4 ng recombinant Ku ( -Ku+rKu) human (HeLa) cell extracts, or 10 g parental (K1), Ku deficient (xrs6), or complemented (xrs6+Ku80) rodent (CHO) cell extracts. a, b, Activity a, Radiolabelled 1 nM 40-bp substrate with a 5′ penultimate abasic site (AP-DSB) (open circle, lanes 1–8) or lyase-resistant analogue (tetrahydrofuran (THF); filled circle, lanes 9–11) was shown to be a 5′-dRP/AP lyase involved in double-strand break repair (19). In further screening for proteins that are reactive at AP sites, we used circular AP site-containing DNA to exclude interferencebyKu80.Circulardouble-strandedDNAwassynthe-sized, using single-stranded M13 DNA as a template, in the pre- N -glycosylase activity releases damaged purines, including 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 8-oxo-7,8-dihydroguanine (8oxoG), generating an AP site. The AP lyase activity cleaves an AP site, via β and δ-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini. See more details on Bioz 定义 中文名称：AP裂合酶 英文名称：AP lyase 其他名称：无嘌呤嘧啶裂合酶;AP核酸内切酶(AP endonuclease) 定义：编号：EC 4.2.99.18。 。通过β消去反应使DNA链中无嘌呤或
Edit keyword. DNA glycosylases (EC 3.2. 2.) are a family of enzymes involved in
8-oxo-dG glycosylase/AP lyase activity in crude mitochondrial extracts was measured by an oligonucleotide incision assay, as previously described (9).
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Consistent with other studies 14 , 15 (reviewed in 16 ) an alternative to NHEJ (Alt-NHEJ) allows for significant joining of undamaged ends in Ku70 deficient cells ( Figure 1d , columns 1 and 2, 7 and 8). Mammalian cells require non-homologous end joining (NHEJ) for the efficient repair of chromosomal DNA double-strand breaks. A key feature of biological sources of strand breaks is associated nucleotide damage, including base loss (abasic or apurinic/apyrimidinic (AP) sites). At single-strand breaks, 5'-terminal abasic sites are excised by the 5'-deoxyribose-5-phosphate (5'-dRP) lyase activity Kevin G. Pinz, Daniel F. Bogenhagen By virtue of its binding to AP sites, PARP-1 could be poised for its role in base excision repair, pending DNA strand incision by APE1 or the 5′-dRP/AP lyase activity in PARP-1. The capacity of human poly(ADP-ribose) polymerase-1 (PARP-1) to interact with intact apurinic/apyrimidinic (AP) sites in DNA has been demonstrated. The Ku AP lyase activity is also strongly suppressed by as little as two paired bases 5′ of the abasic site. Importantly, in vitro end joining experiments show that abasic sites significantly embedded in double-stranded DNA do not block the NHEJ ligation step.